peripheral blood leukocytes  (TaKaRa)

Journal: Frontiers in Medicine

Article Title: Multisystem injury after wasp stings in the Qinling range, Shaanxi, China: clinical profile and independent predictors of poor outcome

doi: 10.3389/fmed.2025.1716614

Figure Lengend Snippet: The expression of key enzyme genes for glucose metabolism. (A–E) The mRNA expressions of GLUT1, GK, PKM2, LDH and PDK1 in the peripheral blood leukocytes of patients in different groups were detected by RT-qPCR. (F) The level of LAC in the serum was evaluated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, or ns p > 0.05.

Article Snippet: Briefly, total RNA was isolated from human peripheral blood leukocytes using RNAiso Plus reagent (Code No.9109, Takara) according to the manufacturer’s protocol.

Techniques: Expressing, Quantitative RT-PCR

Journal: Frontiers in Medicine

Article Title: Multisystem injury after wasp stings in the Qinling range, Shaanxi, China: clinical profile and independent predictors of poor outcome

doi: 10.3389/fmed.2025.1716614

Figure Lengend Snippet: The expression of key enzyme genes for lipid metabolism. (A–F) The mRNA expressions of ACC, ACO, CPT1, FABPpm, FAS and SREBP1c in the peripheral blood leukocytes of patients in different groups were detected by RT-qPCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, or ns p > 0.05.

Article Snippet: Briefly, total RNA was isolated from human peripheral blood leukocytes using RNAiso Plus reagent (Code No.9109, Takara) according to the manufacturer’s protocol.

Techniques: Expressing, Quantitative RT-PCR

Journal: medRxiv

Article Title: A Unified Multiplex Digital PCR-based Platform for Multi-class Biomarker Quantification from Low-volume Blood

doi: 10.1101/2025.08.31.25334810

Figure Lengend Snippet: Development of multiplexed mRNA detection system in FluoMag-dCoDe. (A) Fluorescence-codes for each mRNA target, including three “bacterial genes” and five “viral genes”, are designed by varying the number of universal HEX and FAM TaqMan probe binding sites. (B) Aggregated standard curves show good linear relationship between human total RNA inputs and ddPCR signal outputs. Each datapoint represents a mean ± standard deviation from three replicates. (C) Pearson correlation analysis with r=0.75 and p<0.0001 indicates a good correlation between the readouts from FluoMag-dCoDe and those from gold-standard RT-qPCR for each target. The shaded area represents the standard error of the slope estimate. (D) Two-dimensional FAM-versus-HEX amplitude plot of the multiplex reaction (50 ng total RNA) shows eight target-specific droplet populations and a single negative population. (E) Linear regression curves (n=3) from singleplex (solid) and multiplex (dashed) assays for each target show no significant difference in both slopes and intercepts.

Article Snippet: The Human Blood Peripheral Leukocytes Total RNA was purchased from Takara Bio USA, Inc. (Mountain View, CA, USA).

Techniques: Fluorescence, Binding Assay, Standard Deviation, Quantitative RT-PCR, Multiplex Assay

Journal: medRxiv

Article Title: A Unified Multiplex Digital PCR-based Platform for Multi-class Biomarker Quantification from Low-volume Blood

doi: 10.1101/2025.08.31.25334810

Figure Lengend Snippet: Multiplexed, multi-analyte biomarker detection in a single ddPCR reaction. (A) Two-dimensional amplitude plot resolving eight mRNA and three protein biomarkers in one ddPCR reaction. Inputs: 25 ng human total RNA; CRP 1000 pg/mL, IP10 500 pg/mL, TRAIL 100 pg/mL (spiked in UniQ Buffer). (B) Comparison of ddPCR signal between the integrated assay and individual mRNA or protein assays at identical target inputs as in panel (A), demonstrating preserved quantification accuracy (n=3). (C) Pearson correlation of mRNA signals from the integrated versus mRNA-only assay across varying human total RNA inputs of 100, 50, 25, and 12.5 ng. The integrated assay contains the fixed protein concentration identical to panel (A). (D) Pearson correlation of protein signals for two-fold serial dilutions (CRP: 1000–62.5 pg/mL, IP10: 500–31.25 pg/mL, TRAIL: 100–6.25 pg/mL) between the integrated and protein-only assay. The integrated assay contains a fixed human total RNA input of 25 ng. For panel (C) and (D), each data point represents one reaction. The shaded area represents the standard error of the slope estimate.

Article Snippet: The Human Blood Peripheral Leukocytes Total RNA was purchased from Takara Bio USA, Inc. (Mountain View, CA, USA).

Techniques: Biomarker Discovery, Comparison, Protein Concentration

Journal: medRxiv

Article Title: A Unified Multiplex Digital PCR-based Platform for Multi-class Biomarker Quantification from Low-volume Blood

doi: 10.1101/2025.08.31.25334810

Figure Lengend Snippet: Performance of the eleven-plex FluoMag-dCoDe from low-volume blood samples. (A) Total RNA yield from whole blood samples (n=3) with varying input volumes using an optimized extraction protocol. (B) Plasma recovery volumes as a function of blood input volumes from multiple samples (n=3) following centrifugation-based separation. (C) Fluorescence-coding ddPCR outputs for each biomarker across three blood samples using 10 μL and 25 μL blood for mRNA and protein detections, respectively. Each sample was tested three times. (D) Correlation analysis between mRNA quantification results from FluoMag-dCoDe and those from single-plex RT-qPCR show good concordance. Each datapoint refers to the average value from three replicates for one blood sample. The shaded area represents the standard error of the slope estimate. (E) Protein quantification comparison between FluoMag-dCoDe and ELISA across three blood samples. TRAIL was not detectable in ELISA due to limited sensitivity.

Article Snippet: The Human Blood Peripheral Leukocytes Total RNA was purchased from Takara Bio USA, Inc. (Mountain View, CA, USA).

Techniques: Extraction, Clinical Proteomics, Centrifugation, Fluorescence, Biomarker Discovery, Quantitative RT-PCR, Comparison, Enzyme-linked Immunosorbent Assay